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. 2005 Oct;73(10):6962–6973. doi: 10.1128/IAI.73.10.6962-6973.2005

FIG. 5.

FIG. 5.

Detection of total MMP-2 and proMMP-9 by antigen capture EIA. (A) These data represent the mean nanograms (ng) of total MMP-2 per mg protein in upper urogenital tissue homogenates derived from C57BL/6 (black bars) or C3H/HeN (gray bars) mice plus or minus the standard deviation (error bars). There was no significant elevation in total MMP-2 by this assay in either strain at any time point postinfection relative to day 0. However, total MMP-2 was elevated in C3H/HeN relative to C57BL/6 mice on days 0, 21, and 28 postinfection (P < 0.04 in each case by a two-way t test). (B) These data represent the ng of proMMP-9 per mg total protein in the same upper genital tract homogenates shown for total MMP-2 in panel A. Significant elevation in proMMP-9 relative to day 0 was first observed in C57BL/6 mice at day 4 and for C3H/HeN mice at day 7 and persisted through day 56 for both strains (P < 0.05 by a two-way t test in each case). A significant elevation of proMMP-9 in the C57BL/6 mice relative to the C3H/HeN mice was observed on day 21 postinfection (P < 0.02).