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Immunoblot analysis of PNAG expression by strains of S. aureus (listed along the left-hand side of the blot) evaluated in animal models of infection. Undiluted and 1:10 diluted boiled EDTA extracts from 5 × 109 cells were applied by a vacuum manifold to the membrane which was probed with 200 ng/ml of affinity-purified goat antibody raised to a conjugate of dPNAG and diphtheria toxoid (43) followed by swine anti-goat immunoglobulin G conjugated to horseradish peroxidase.
