TABLE 3.

Primer sequences to amplify vtp-specific probes and ORF fragments lacking the signal sequence for cloning into the BamHI site of pET-15b

Isolate	Sequence (5′ to 3′)	Size of probe (bp)
For probes
BAK-1	GGTAAGAAGATAGTTGCTGGTGGTGC	260
BAK-2	TGGCTTTTTTTGCATTTGCATCAG
DAH-1	GATGAGCTTGCTAAAGCTATTGGACAG	324
DAH-2	TGTATTTAACTTACCAAGTTCTTCAGCTCCC
FRO-1	CGCTATTGGAAAGAAAATTAAAGAAGATG	265
FRO-2	GCTTTTTGTGCATTCTCATCAGTAGC
HAN-1	GCTATTAAAAAGAAAATTCAAGCAGATGGTC	277
HAN-2	ATCTATGGCTTCCTTTGCATTAGCAC
YOR-1	GCTAAAGCTATTGGGAAAAAAATTGATC	327
YOR-2	TTCAACTTACCAAGCTCTTCAGCTCC
For cloning
Vtp1C	GGATCCGTGTAATAATGGAGGCCCAGAG
Vtp1G	GGATCCGTGTAATAATGGAGGGCCAGAG
Vtp2A	GGATCCTTAAGGTTTAACAGGGGTCGC
Vtp2G	GGATCCTTAAGGTTTAGCAGGGGTCGC