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RT-PCR analysis of the TC genes of Y. enterocolitica T83. Expression of tcbA (lanes 2 to 7), tcaC (lanes 8 to 13), and tccC (lanes 14 to 19) was assessed at 30°C (lanes 2, 8, and 14) and at 37°C (lanes 4, 10, and 16). Lanes: 1 and 20, molecular weight markers; 3, 5, 9, 11, 15, and 17, reverse transcriptase-negative controls; 6, 12, and 18, Y. enterocolitica T83 genomic DNA-positive controls; 7, 13, and 19, template-negative controls.
