FIG. 2.

Induction of Omp31-specific CTLs in spleen cells from pCIOmp31-immunized mice. Cytotoxicity was detected in a standard 6-h ⁵¹Cr release assay. Target cells were A20JpCI (dashed lines) or A20JOmp31 (solid lines) cells labeled with ⁵¹Cr. Effector cells were the splenocytes from immunized mice previously cultured for 5 days with mitomycin C-treated A20JOmp31.The cytotoxicity was measured at the indicated effector/target cell ratios. Each value represents the mean ± SD of the responses of spleen cells from five individual mice. Data are representative of three separate experiments.