FIG. 4.

pCIOmp31 elicits effector CD8⁺ T cells that mediate cytotoxicity via perforin and CD4⁺-specific T cells that use the Fas-FasL pathway. 51Cr release CTL assay was performed as described for Fig. 2. Target cells were A20JOmp31 cells. The effector/target cell ratio was 100:1. pCIOmp31-specific effector cells were depleted of CD4⁺ T cells (−CD4⁺) or CD8⁺ T cells (−CD8⁺) by using mouse CD4 (L3T4) Dynabeads or mouse CD8 (Lyt2) Dynabeads or were not depleted (total cells). Depleted and nondepleted effector cells were preincubated for 2 h with RPMI (-), brefeldin A (BFA), or concanamycin A (CCA). Each value represents the mean of triplicates ± SD of the response of a pool of spleen cells from five mice. Data are representative of two separate experiments.