TABLE 3.
Expansion of CD45RO+ subsets following antigenic restimulationa
| Stimulation | Group (n = 4) | Mean cell no. (±SEM) by CD45RO+ cell subset
|
||
|---|---|---|---|---|
| Ungated | CD4+ | CD8+ | ||
| rESAT-6-CFP10 | Infected | 3,139 (484)** | 484 (142)* | 385 (59)** |
| Control | 560 (277) | 101 (34) | 26 (10) | |
| M. bovis PPD | Infected | 3,740 (512)** | 1,426 (494)* | 303 (55)** |
| Control | 257 (155) | 49 (30) | 12 (9) | |
a
PBMCs from M. bovis-infected cattle (n = 4) or control cattle (n = 4) were stained with PKH67; cultured with medium alone, rESAT-6-CFP10 (10 μg/ml), or M. bovis PPD (5 μg/ml) for 6 days; stained for CD4, CD8, and CD45RO; and analyzed for proliferation. Data represent the mean (±SEM) numbers of cells that had proliferated per 10,000 PBMCs minus values from nonstimulated (medium alone) cultures. A single asterisk indicates a significant difference (P < 0.05) from M. bovis-infected animals compared to noninfected control animals. A double asterisk indicates a significant difference (P < 0.01) from M. bovis-infected animals compared to noninfected control animals.