TABLE 1.
Viable bacteria recovered from mice that were vaccinated with clone pool DNA (trial 1) and challenged with live M. avium subsp. paratuberculosisa
| Immunization group | Spleen
|
Mesenteric lymph nodes
|
||||
|---|---|---|---|---|---|---|
| CFU/mgb | SEM | P valuec | CFU/mgb | SEM | P valuec | |
| No DNA, noninfected | 0.0 | 0.0 | 0.0 | 0.0 | ||
| No DNA, infected | 2,047.3 | 478.2 | 1,240.1 | 218.0 | ||
| Microcarrier, infected | 1,422.6 | 264.6 | 0.2680 | 1,538.5 | 298.5 | 0.4295 |
| Vector DNA, infected | 1,604.6 | 268.0 | 0.4299 | 1,246.5 | 270.2 | 0.9855 |
| Clone pool 1 | 27.2 | 26.3 | 0.0001 | 63.5 | 62.4 | 0.0001 |
| Clone pool 2 | 11.4 | 8.9 | 0.0001 | 41.2 | 33.1 | 0.0001 |
| Clone pool 3 | 1,470.0 | 253.6 | 0.3004 | 948.7 | 231.1 | 0.3712 |
| Clone pool 4 | 2,377.7 | 628.2 | 0.6804 | 911.4 | 270.4 | 0.3565 |
| Clone pool 5 | 1,436.7 | 452.9 | 0.3662 | 714.6 | 201.0 | 0.0933 |
| Clone pool 6 | 1,578.9 | 224.2 | 0.3869 | 1,543.7 | 392.5 | 0.5075 |
| Clone pool 7 | 793.6 | 432.2 | 0.0679 | 1,057.2 | 885.6 | 0.8433 |
| Clone pool 8 | 724.4 | 385.5 | 0.0451 | 281.5 | 162.7 | 0.0024 |
| Clone pool 9 | 1,130.3 | 416.7 | 0.1655 | 1,441.9 | 771.9 | 0.8024 |
| Clone pool 10 | 144.2 | 93.7 | 0.0010 | 247.4 | 174.7 | 0.0023 |
| Clone pool 11 | 2,754.2 | 399.5 | 0.2714 | 3,239.0 | 729.4 | 0.0171 |
a
BALB/c mice were immunized with clone pool DNA (2 μg), boosted 3 weeks later, and challenged intraperitoneally with live M. paratuberculosis (strain 6112; 108 CFU). Mice were necropsied after 3 months of infection, and tissues were processed for culture.
b
Mean CFU/mg tissue (n = 10).
c
The values in boldface type are P values for groups that were significantly different than the no-DNA, infected control group (P < 0.05).