TABLE 3.

Recovery of viable bacteria from tissues of mice vaccinated with clone array DNA (trial II) and challenged with live M. avium subsp. paratuberculosis^a

Immunization group	Spleen			Mesenteric lymph nodes
	CFU/mgb	SEM	P valuec	CFU/mgb	SEM	P valuec
No DNA, noninfected	0	0		0	0
No DNA, infected	242.49	93.51		804.21	638.61
Parent clone pool	723.41	199.83	0.1257	649.91	228.32	0.5896
Nonrepetitive clones	764.88	363.74	0.6272	142.52	38.31	0.0776
Clone array 1	2.74	0.68	0.0007	15.89	8.23	0.0106
Clone array 2	1.34	0.33	0.0003	0.28	0.13	0.0003
Clone array 3	453.08	411.48	0.5599	39.29	36.13	0.0548
Clone array 4	28.88	27.76	0.0144	10.89	3.91	0.0044
Clone array 5	10.91	10.5	0.0029	49.9	32.69	0.0636
Clone array 6	422.96	182.77	0.8027	700.37	471.77	0.4425
Clone array 7	407.82	240.27	0.6697	336.98	83.79	0.4665
Clone array 8	61.47	32.78	0.0379	46.94	27.11	0.0534
Clone array 9	13.43	11.83	0.0024	18.65	12	0.0116
Clone array 10	31.61	15.53	0.0118	89.95	54.76	0.1212

^aBALB/c mice were immunized with clone pool DNA (2 μg), boosted 3 weeks later, and challenged intraperitoneally with live M. paratuberculosis (strain 6112; 10⁸ CFU). Mice were necropsied after 3 months of infection, and tissues were processed for culture.

^bMean CFu/mg tissue (n = 10).

^cThe values in bold-face type are P values for groups that were significantly different than the no-DNA, infected control group (P < 0.05).