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mRNA clonal expression of rickettsial genes in Salmonella after overnight incubation. (A) RT-PCR using primers specific for tlyC. (B) RT-PCR using primers specific for pld. Lane 1, negative control (RT-PCR using RNA extracted from SB109); lane 2, recombinant Salmonella RNA expressing tlyC (A) or pld (B); lane 3, RT-PCR of RNA with no reverse transcriptase enzyme.
