TABLE 1.
GroEL cannot be detected on the surface of Brucella spp.
| Labeling condition | Species or material
|
||||||
|---|---|---|---|---|---|---|---|
| B. suis | B. abortus | B. suis virB2 mutant | B. suis virB4 mutant | B. suis ΔmanB | B. suis stresseda | GroEL-labeled latex beads | |
| GroEL MAbb | − | − | − | − | + | − | + |
| Secondary Abc | − | − | − | − | + | − | − |
| PrPd | − | − | − | − | + | − | ND |
| Ribonucleasee | − | − | − | − | + | − | ND |
a
B. suis organisms were stressed for 3 min at 45 °C.
b
Anti-Escherichia coli GroEL mouse monoclonal antibody 9A1/2 was used in association with secondary anti-mouse antibody (tetramethyl rhodamine isothiocyanate conjugated).
c
The secondary antibody was used alone for labeling under the same conditions as described in footnote b.
d
Recombinant hamster PrP labeled as described in Materials and Methods.
e
Ribonuclease labeled as described in Materials and Methods.