FIG. 2.

Representative experiment showing confirmation of gene expression patterns in primed (pTv) and control (T. vaginalis [Tv]) parasites by semiquantitative RT-PCR analyses. Total RNA from pTv and T. vaginalis was isolated as detailed in Materials and Methods. RNA was reverse transcribed using oligo(dT) primer, and PCR was performed using gene-specific primers (Table 1). (A) RT-PCR products separated on EtBr-stained gels after electrophoresis in 2% agarose. (B) Gene expression pattern relative to the housekeeping trichomonad α-tubulin gene used as a control. The values were obtained by scanning the intensities of bands from pictures of the gels in A using the Scion image beta program. The expression for each gene was relative to baseline density for α-tubulin plotted on the graph. This experiment was repeated on four separate occasions with similar results.