FIG. 4.
Keratinocytes demonstrate antimicrobial activity against S. aureus that is partially dependent on cathelicidin expression. Bacterial recovery at 3, 4, and 5 h following incubation of log-phase S. aureus ΔmprF (inoculum, 104 CFU/ml) with isolated Cnlp+/+ and Cnlp−/− mouse KC is shown. “No KC” control wells were incubated with bacteria grown under identical conditions. Data are means ± standard errors from at least three independent experiments performed in triplicate. Statistical analysis was performed using the paired Student t test. P < 0.001 for Cnlp+/+ KC versus No KC at 3, 4, and 5 h; P < 0.02 for Cnlp+/+ KC versus Cnlp−/− KC at 3, 4, and 5 h.