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Coomassie-stained SDS-PAGE gel. Lane 1, molecular mass standards in kilodaltons; lane 2, outer membrane preparation of M. catarrhalis 25240; lane 3, whole-cell lysate of E. coli containing pCATCH6 preinduction; lane 4, whole-cell lysate of E. coli containing pCATCH6 after 4-h induction with IPTG; lane 5, purified recombinant OMP G1a. The arrow on the left indicates the native OMP G1 band in lane 2. The arrow on the right indicates the recombinant OMP G1a in lanes 4 and 5.
