Figure 1.

Brain metastasis burden quantification methods

(A) Bioluminescent signal detected by IVIS in mice with brain metastases established upon injection of luciferase expressing melanoma cells.

(B) Pictures of brains from mice in Figure 1A.

(C) Image of a mouse brain with metastases generated upon injection of tdTomato expressing FCT melanoma cells.

(D) Schematic representation of the process followed to generate brain metastases, sample processing and quantification of the metastatic burden.