Figure 7.
Expected results: Sample phenotyping
(A) Fluorescence image of a brain metastasis edge depicting microglia stained with anti-GFP antibody (green) and anti-Ki67 (red) showing the nuclei of dividing cells. DAPI stains cell nuclei (blue).
(B) Fluorescence image of a brain metastasis edge depicting microglia stained with anti-P2RY12 antibody (green) and astrocytes labeled with anti-GFAP (red). DAPI stains cell nuclei (blue).
(C) Fluorescence image of a metastasis edge depicting astrocytes labeled with anti-GFAP (green) and microglia stained with anti-TMEM119 antibody (red). DAPI stains cell nuclei (blue).
(D) Fluorescence image of a metastasis edge depicting microglia stained with anti-TMEM119 antibody (green), dividing cell nuclei with anti-KI67 (red) and astrocytes labeled with anti-GFAP (magenta DAPI stains cell nuclei (blue). White bar scale = 20 μm.
