Figure 8.

Troubleshooting 2 and 3

(A) Picture of a good quality scanned slide.

(B) Picture of an acceptable quality scanned slide with bubbles within the Mowiol mounting medium.

(C) Picture of a non-acceptable quality scanned slide where Mowiol has shrink.

(D) Picture of a histogram from the software Zen Blue for the Axioscan Z1 controller showing the fluorescence and light histogram. The top panel shows a histogram where light information is scattered outside the histogram and therefore a bad example. The bottom picture represents a good example.