TABLE 1.
Change (n-fold) in fluorescence upon infection with P. aeruginosa as measured by cDNA-spotted microarrays in quadruplicate
| Gene or gene producta | WT CFTR | ΔF508 CFTRb | I.M.A.G.E. no.c |
|---|---|---|---|
| IL-6* | 6.61 (±5.50) | NC | 310406 |
| ICAM-3 (CD50) | 6.20 (±1.88) | NC | 754080 |
| Low-affinity immunoglobulin | 4.52 (±4.31) | NC | 51447 |
| Gamma Fc receptor III-1 precursor | |||
| Endothelin 3 | 4.32 (±4.78) | NC | 66532 |
| IL-8* | 4.00 (±2.14) | NC | 549933 |
| IL-8* | 3.57 (±1.63) | NC | 549933 |
| Fibroblast growth stimulatory factor 7 (keratinocyte growth factor) | 3.45 (±2.44) | NC | 365515 |
| Intercellular adhesion molecule 1 (CD54) | 3.13 (±1.85) | NC | 145112 |
| IL-6* | 3.05 (±1.31) | NC | 310406 |
| EGLN3 | 2.69 (±1.58) | NC | 122982 |
| CXCL1 (GRO1 oncogene) | 2.44 (±0.66) | NC | 323238 |
| Plasminogen activator inhibitor type II (arginine-serpin) | 2.23 (±0.60) | NC | 70692 |
| Cytochrome P450 IB1 (dioxin inducible) | 3.70 (±1.07) | 3.44 (±0.82) | 782760 |
| Transforming growth factor beta 1 precursor | 3.67 (±1.60) | 3.04 (±1.10) | 136821 |
| Human c-jun proto-oncogene (JUN), clone hCJ-1 | 3.59 (±1.32) | 2.75 (±0.81) | 358531 |
| Transforming protein RHOB | 3.40 (±0.82) | 3.71 (±1.56) | 768370 |
| Complement component 8, beta polypeptide | 3.11 (±0.78) | 3.20 (±0.59) | 83210 |
| Activating transcription factor 3 | 2.42 (±0.97) | 2.32 (±0.69) | 51448 |
| Stanninocalcin 2 | NC | 2.19 (±0.51) | 130057 |
a
*, For both IL-6 and IL-8, the same cDNA clone was represented twice on the array. Both sets of results are given for each gene product.
b
NC, no change (i.e., change of less than twofold detected).
c
I.M.A.G.E., Integrated Molecular Analysis of Genomes and Expression consortium.