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. 2005 Oct;73(10):7069–7073. doi: 10.1128/IAI.73.10.7069-7073.2005

FIG. 2.

FIG. 2.

(A) Production of IL-1β, IL-6, TNF-α, IFN-γ, and IL-2 by PBMC in response to SEB alone (SEB), SEB plus edema toxin incubated simultaneously (SEB+Edema Toxin), or pretreatment of cells with edema toxin for 3 h before SEB [(Edema Toxin)+SEB]. Values are the means ± SD for two PBMC samples and repeated experiments for each panel. (B) Production of MCP-1, MIP-1α, and MIP-1β by PBMC in response to SEB alone, SEB plus edema toxin incubated simultaneously, or pretreatment of cells with edema toxin for 3 h before SEB. (C) Production of IL-1β, IL-6, TNF-α, IFN-γ, and IL-2 by PBMC in response to SEB alone, SEB plus edema toxin incubated simultaneously, or SEB preincubated for 3 h with cells before edema toxin posttreatment [SEM+(Edema Toxin)]. (D) Production of MCP-1, MIP-1α, and MIP-1β by PBMC in response to SEB alone, SEB plus edema toxin incubated simultaneously, or SEB preincubated for 3 h before edema toxin posttreatment. The SEB, EF, and PA concentrations for all of these experiments are 7 nM, 5 nM, and 12.5 nM, respectively. *, P < 0.05 compared with cells treated with SEB alone.