Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2025 Feb 25;27(6):1443–1457. doi: 10.1093/neuonc/noaf055

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2025. Published by Oxford University Press on behalf of the Society for Neuro-Oncology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.

PMC Copyright notice

CRISPRoff repression of MGMT sensitizes GBM cells to TMZ long term. — CRISPRoff repression of MGMT sensitizes GBM cells to TMZ long term. (A) Cell viability dose–response curves for temozolomide (TMZ) sensitivity in LN18 (left) and T98G (right) polyclonal populations at the indicated number of days following delivery of CRISPRoff and the indicated sgRNA(s). IC50 is calculated for each condition. (B) Cell viability dose–response curves for temozolomide (TMZ) sensitivity in LN18 (left) and T98G (right) intermediate and long-term monoclonal populations at the indicated number of days following delivery of CRISPRoff and the indicated sgRNA(s). IC50 is calculated for each condition using 3 parameter nonlinear fit on [inhibitor] versus response.