FIG. 2.

Adjuvant effect of FljB on the plasma IgG subclass responses to SBR. Mice were immunized by the i.n. route on days 0 and 18 with 20 μg SBR or 20 μg SBR coadministered with 5 μg FljB. Wild-type mice receiving PBS via the i.n. route served as controls. SBR-specific plasma IgG, IgG1, IgG2a, and IgG2b responses were analyzed 2 weeks following the second i.n. immunization (day 32). Results represent the arithmetic mean ± SEM of eight mice per group. * and *** indicate significance differences between the groups of mice at P < 0.05 and P < 0.001, respectively.