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Selection of AMA1-binding peptides. (A) Equal amounts of phage (1 × 1011 CFU/ml) after each round of panning (round 0 [Rd 0] to Rd 4) binding to immobilized PfAMA1. Phage displaying peptides with an affinity for AMA1 are enriched after round 4. (B) Pooled phages, after the fourth round of panning, were incubated with wells coated with PfAMA1, PcAMA1, RESA, BSA, or MAb 1F9 or left uncoated. Round-4 phages are specific for PfAMA1.
