FIG. 7.

Macrophage cytokine response to Salmonella infection and LPS challenge. Wild-type (Wt) and CCL2^−/− mouse peritoneal macrophages were isolated, plated at a density of 5 × 10⁶/well, and incubated with S. enterica serovar Typhimurium SL1334 (at a multiplicity of infection of 10) or with 1 μg/ml Salmonella LPS. Medium was collected at various time points, and the supernatants were analyzed for production of TNF-α (A) and IL-6 (B). These data are representative of three identical experiments. An asterisk denotes P < 0.05 for CCL2^−/− mouse macrophages infected with salmonellae compared to wild-type macrophages infected with salmonellae. A double asterisk denotes P < 0.05 comparing CCL2^−/− mouse macrophages to wild-type mouse macrophages incubated with Salmonella LPS.