FIG. 1.

RT-PCR and Western blot analysis. RNA and whole-cell extracts from wild-type strain Y-transfected, pTEX-transfected (YpTEX), or YpTEX-CD40L-transfected (YpTEX-CD40L) epimastigotes were used for RT-PCR (A) and Western blot analysis (B). Trypomastigotes derived from these epimastigotes were used for RT-PCR (C) and Western blot analysis (D). Specific primers were used to amplify the cDNA indicated (neo, neomycin phosphotransferase). Whole-cell extracts (WE) or supernatants (S) from YpTEX- and YpTEX-CD40L-transfected trypomastigotes were analyzed by Western blotting (E). For protein analysis, CD40L was detected using a specific antibody, and an anti-TcRGG polyclonal antibody was used as a control.