FIG. 3.

Proliferation SCs activated with T. cruzi lysates and production of IFN-γ by SCs from mice infected with the Y, YpTEX, and YpTEX-CD40L strains. Three groups of mice (66 mice per group) were infected on day 0 with the Y (solid bars), YpTEX (diagonally cross-hatched bars), and YpTEX-CD40L (open bars) strains. One group of five mice (horizontally cross-hatched bar) was not infected and served as a control (day 0 postinfection). Every week, five mice were sacrificed. (A) SCs were harvested and incubated with T. cruzi lysate. (B) IFN-γ was measured by ELISA in the culture supernatants of SCs stimulated with T. cruzi lysates. The data are means and standard errors of the means of three independent experiments. For comarisons of YpTEX-CD40L and YpTEX, one asterisk indicates that the P value is <0.05 and two asterisks indicate that the P value is <0.01 (as determined by the Mann-Whitney U test).