Skip to main content
. 2025 Jul 16;18(7):dmm052148. doi: 10.1242/dmm.052148

Fig. 7.

Fig. 7.

Lymphatic capillary coverage in lumbar spinal cord and EDL muscle appears largely unaltered in SOD1-G93A mice. (A) Representative cross-section of p120 NC EDL muscle stained for Lyve1. Scale bar: 200 μm. Inset: higher-magnification view. Scale bar: 50 μm. (B) Representative cross-section of p120 ALS EDL muscle stained for Lyve1. Scale bar: 200 μm. Inset: higher-magnification view. Scale bar: 50 μm. (C) Lyve1 density (Lyve1 staining area/area of cross-section×100%) (n=4 males/genotype; P=0.6875). (D) Vessel profile area (μm2). (E) Muscle tissue clearing before (top left) and after (bottom left) iDISCO protocol. Representative lightsheet slice of p126 NC EDL tissue labeled for Lyve1 (left, raw Lyve1 staining; middle, thresholded Lyve1 signal; right, MIP of thresholded Lyve1 image). Scale bar: 1 cm. (F) Representative lightsheet slice of p126 NC lumbar spinal cord tissue labeled for Lyve1 (left, raw Lyve1 staining; middle, thresholded Lyve1 signal; right, MIP of thresholded Lyve1 image). Scale bar: 1 cm. (G) Lyve1 density in thresholded lightsheet images in EDL tissue (n=6 males/genotype; P=0.1597). (H) Lyve1 density in thresholded lightsheet images in spinal cord tissue (n=6 males/genotype; P=0.2784). All means in C, D, G and H compared by unpaired two-tailed t-test.