TABLE 1.
Antigens identified by immunoscreening of the L. donovani AG83 cDNA expression library with sera of kala-azar patients from Bihar, India
| Antigen | Highest sequence identity (description) | GenBank accession no.a | No. of analyzed primary plaques | No. of corresponding sera in primary immunoscreens |
|---|---|---|---|---|
| LDHSP70 | 71.2-kDa L. donovani heat shock protein LDHSP70 (X52314) | AY702003 | 4 | 4 |
| HASPB1 | 42.2-kDa L. donovani hydrophilic cell surface protein HASPB1 (AJ011810) | AY702004 | 3 | 3 |
| LePa2 | 25.1-kDa L. infantum 20 S proteasome α-2 subunit LePa (Y13059) | AY702005 | 3 | 3 |
| P0 | 35-kDa L. chagasi 60 S ribosomal protein P0 (L29300) | AY702006 | 3 | 3 |
| EF1B | 25.6-kDa Leishmania major putative elongation factor 1β LmjF34.0820 | AY702007 | 1 | 1 |
| ARP-1 | 10.4-kDa L. donovani acidic ribosomal protein ARP-1(LiP, P2β; AF034539) | AY702008 | 4 | 2 |
| α-Tubulin | 49.7-kDa L. donovani α-tubulin (U09612) | AY702009 | 8 | 2 |
| LDAG1 | 33.1-kDa L. major hypothetical protein LmjF36.0540 | AY702010 | 2 | 1 |
| LDAG2 | 14.5-kDa L. major hypothetical protein LmjF30.0770 | AY702011 | 1 | 1 |
| LDAG3 | 28.8-kDa L. major hypothetical protein LmjF34.2700 | AY702012 | 1 | 1 |
a
The sequences of the inserts coding for the Leishmania donovani antigens were submitted to GenBank.