REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-CD41-PE	Beckman Coulter	Cat#A07781; clone P2
Anti-CD14-FITC	Beckman Coulter	Cat#B36297
Anti-CD16-PC5	Beckman Coulter	Cat#A07767; clone 3G8
Rabbit polyclonal anti-Alix 1:50	Novus Biologicals, Bio-Techne	Cat#NBP1-49701; RRID: AB_10011818; lot: D10 6916-8
Mouse monoclonal anti-CD45 1:250	BD Transduction Laboratories	Cat#610266; RRID: AB_397661; clone 69/CD45
Rabbit polyclonal anti-CD42a 1:10	GeneTex	Cat#GTX32502; lot:822202558
Rabbit polyclonal anti-Stx2a 1:50	Dr Stefano Morabito, ISS Rome	N/A
Mouse monoclonal anti-C3 1:10	Hycult Biotech	Cat#HM2394; clone C3-42.3
Mouse monoclonal anti-C9 1:10	Hycult Biotech	Cat#HM2264; RRID: AB_10502672; clone WU 13-15
Anti-Mouse Secondary Antibody (∗kit component)	ProteinSimple, Bio-Techne	lot: 105741
Anti-Rabbit Secondary Antibody (∗kit component)	ProteinSimple, Bio-Techne	lot: 105824
Bacterial and virus strains
C600-933W strain	Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD, USA	N/A
Biological samples
Human blood	Emilia-Romagna Regional Blood Centre, Maggiore Hospital, AUSL Bologna, Italy	N/A
Chemicals, peptides, and recombinant proteins
Bio-Rad protein assay	Bio-Rad	Cat#500-0006
Folin-Ciocalteu’s phenol reagent	Merck	Cat#F9252
Penicillin-Streptomycin	Cambrex	Cat#DE17-602E
L-glutamine	Sigma-Aldrich	Cat#G7513
FBS	Lonza	Cat #DE14-701E
Ficoll-Paque	Merck	Cat#GE17-1440-02
Leucine, L-[4,5-3H] radionuclide	PerkinElmer	Cat#NET1166001MC
Trichloroacetic acid	Carlo Erba	Cat#411527
Fibrin polymerization inhibitor Gly-Pro-Arg-Pro (GPRP)	Merck	Cat#G1895-5MG
cOmplete protease inhibitor cocktail	Sigma-Aldrich	Cat#11836170001
RPMI 1640 medium	Lonza	Cat#BE12-702S
DMEM High Glucose (4,5 g/L), w/o L-Glutamine, with Sodium Pyruvate	Capricorn Scientific	Cat#DMEM-HPXA
polymyxin B derivative NAB741	Northern Antibiotics	N/A
polymyxin B derivative NAB7061	Northern Antibiotics	N/A
polymyxin B derivative NAB815	Northern Antibiotics	N/A
Cyanogen bromide-activated Sepharose 4B	Pharmacia	N/A
Glycoprotein B1011 (Galα1-4Galβ-O-spacer-BSA)	Glycorex AB	N/A
EZ Standard Pack 1	ProteinSimple, Bio-Techne	Cat#PS-ST01EZ-8
Anti-Mouse Detection Module ∗	ProteinSimple, Bio-Techne	Cat#DM-002; lot: 35507
Anti-Rabbit Detection Module ∗	ProteinSimple, Bio-Techne	Cat#DM-001; lot: 356039
L-Lactate Dehydrogenase (LDHA)	Sigma-Aldrich	Cat#SAE0049
Critical commercial assays
ToxinSensor™ Gel Clot Endotoxin Assay Kit	GenScript	Cat#L00351
EasySep Human Neutrophil Enrichment Kit	Stemcell Technologies	Cat#18001
Cell titer-Glo® luminescent assay	Promega	Cat#G7570
Annexin V-FITC kit	Miltenyi Biotec	Cat#130-092-052
7-AAD BD- Pharmingen	BD-Pharmingen	Cat#559925
Urea assay kit	Abcam	Cat#ab83362
Creatinine assay kit	Abcam	Cat#ab65340
Experimental models: Cell lines
Raji cells	Prof. Andrea Bolognesi, DIMEC, UNIBO. Bologna	N/A
Vero cells	Dr Stefano Morabito, ISS Rome	N/A
Experimental models: Organisms/strains
CD-1 female mice: CD-1® IGS Mouse [Crl:CD1(ICR)], Outbred	Charles River Laboratories	Strain code: 022
Software and algorithms
ReactLab™ Equilibria	Jplus Consulting Pty Ltd	N/A https://www.jplusconsulting.com/Products/
NTA 3.4 analytical software	Malvern Panalytical	N/A
GraphPad Prism 8 software	GraphPad Software	RRID:SCR_002798https://www.graphpad.com/features
Other
NanoSight RS300 system	Malvern Panalytical	N/A
Wes	ProteinSimple, Bio-Techne	Cat#004-600
Spark	Tecan	N/A
Gel-filtration column Sephacryl S-500 HR	Merck	Cat#GE17-0613-01
ActiClean Etox column	Sterogene Bioseparations	Cat#2705
950 spectrophotometer	PerkinElmer	N/A
Lyric cytometer	Becton Dickinson	Cat#BDFACSLyric