Petrified Chichkan sphaeromorph. (A) Digital photograph
of the equatorial plane of the specimen in reflected light, before AFM
analysis. (B) AFM image showing the part of the specimen
analyzed and, denoted by the white rectangles, the areas for which
higher magnification micrographs are shown
(C–F); the irregular black areas are
cavities within and adjacent to the cell wall, produced by acid
etching; the blue line crossing rectangle C shows the transect through
the cell wall along which topography was measured (G).
(C–F) Atomic force micrographs showing the substructure
of the representative portions of the cell wall shown in the
corresponding rectangles in B; the white line in
C denotes the transect (locations 1–15, from outside
the spheroid to beyond its inner surface) where the adhesion
measurements plotted in Fig. 5 were obtained. (G) A
topographic transect through a portion of the cell wall, measured from
inside the spheroid (Left) to beyond its outer surface
(Right) along the blue line that in B
crosses rectangle C, demonstrating the characteristic preferential
etching of the matrix adjacent to both the inner and outer surfaces of
the wall.