FIG. 2.

Lkt binds to the transfectant cell line expressing bovine CD18 fragment encompassing amino acids 1 to 291. The bovine-murine chimeric CD18 transfectant cell lines 6S1 (b291m), 4S1 (m291b527m), and 2S2 (m527b) were tested for Lkt binding by flow cytometry. The parent cell line, P815, and the previously developed transfectant cell line, 2B2, expressing the full-length bovine CD18, were used as the negative and positive controls, respectively. The cells were treated with Lkt, and the binding of Lkt to the cells was detected by treating the cells with FITC-conjugated anti-Lkt MAb MM-605. Results of one representative experiment out of three are shown.