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TX-114 extraction of M. arthritidis indicates activity is restricted to the detergent phase. Following detergent extraction of washed pellets of virulent strain 158p10p9 (see Materials and Methods), aqueous and detergent phases were assayed for induction of TNF-α by RAW 264.7 cells after 24 h. LPS-free NS was similarly treated with TX-114, and the phases were also tested. LPS (10 ng/ml) was a positive control. Mean values ± SEM of triplicate determinations are shown.
