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Effect of polymyxin B on M. arthritidis extracts. Polymyxin B (10 μg/ml final concentration) was incubated with extract (Ex) dilutions from virulent strain 158p10p9 at 200, 40, and 8 ng/ml for 1 h at 37°C prior to being added to RAW 264.7 cells for induction of TNF-α. LPS (100 ng/ml) and lipid A (100 ng/ml) were used as positive controls to show the inhibitory effects of polymyxin B on activation by these inducers.
