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. 2002 Jul 1;99(14):9133–9138. doi: 10.1073/pnas.142277999

Table 1.

DNA polymerase φ activity is stimulated by yeast PCNA

DNA polymerase PCNA addition DNA synthesis*
−Aphidicolin, pmol (fold stimulation) +Aphidicolin, pmol
GST-Pol5p 2.5 (1) 0.5
GST-Pol5p + 12.1 (4.8) 1.3
Polφ 7.0 (1) 0.4
Polφ + 40.0 (5.7) 1.3
Pol5dn 0.1 (1) 0.1
Pol5dn + 0.1 (1) 0.12
Polδ 27.1 (1) 1.4
Polδ + 163.0 (6.0) 2.1
Polɛ 35.2 (1) 0.8
Polɛ + 40.2 (1.1) 0.9
*

DNA synthesis was measured in the presence of poly(dA)500⋅oligo(dT)10 (5:1) as a template-primer. 

Because the purity of the Pol5dn polypeptide was not the same as that of Polφ, the concentration of Pol5dn was estimated by Western blot using mouse polyclonal antibodies against GST-Pol5p, and the same amount of Pol5dn as Polφ was used for the DNA polymerase assay. 

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