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. 2025 Jul 17;13:1617737. doi: 10.3389/fcell.2025.1617737

FIGURE 1.

Panel of visualizations showing UMAP plots and expression data. (A) UMAP of various cell types labeled in different colors. (B-D) UMAP plots comparing control, lesional, and non-lesional samples with a color gradient indicating psoriasis score. (E-H) UMAP plots with a color gradient indicating plakin score. (I) Dot plot showing expression levels of different genes across conditions, with EPPK1 highlighted. (J-L) Violin plots showing EPPK1 expression levels across different cell types in control, lesional, and non-lesional samples, with distinct color coding.

Comprehensive scRNAseq analysis of plakin regulation in psoriasis (A) UMAP visualization from a comprehensive scRNAseq analysis, revealing 12 distinct cell clusters. (B–D) Feature plots illustrating a psoriasis module score, including the expression levels of S100A7A, S100A8, S100A9, IL17A, IL1B, IL22, IFNG, KRT6A, KRT16, MKI67, and IL23A genes. Feature plots are split by condition into (B) (F) control, (C) lesional, and (D) non-lesional psoriasis. (E) UMAP of a subset from the keratinocyte cluster, showing 10 distinct clusters representing stages of keratinocyte differentiation. (F–H) Feature plots showing a plakin module score, including expression levels of DSP, DST, EPPK1, EVPL, MACF1, PLEC, and PPL genes, split by condition into (F) control, (G) lesional, and (H) non-lesional. (I) Dot plot showing expression levels of epidermal plakins in the keratinocyte subset split by condition. Dot size represents the expression percentage for each gene with color intensity reflecting average gene expression levels. (J–L) EPPK1 expression levels as violin plots, split by condition. The mean expression level for each keratinocyte cluster is indicated by a black dot, with the solid black line representing a smooth sixth-degree polynomial regression fit through the means. (J) Grey violins show EPPK1 expression levels in healthy skin, (K) red violins illustrate EPPK1 expression in lesional psoriasis, and (L) orange violins show EPPK1 expression in non-lesional psoriasis.