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. 2025 Jul 17;13:1617737. doi: 10.3389/fcell.2025.1617737

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Copyright © 2025 Kühtreiber, Drexler, Salek, Auer, Griss, Mildner and Fuchs.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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A series of scientific visualizations depicting various data analyses. Image A shows a UMAP scatter plot clustering different cell types. Image B presents another UMAP plot with different cell states. Image C is a dot plot showing gene expression levels and percentages. Image D and E are volcano plots illustrating gene expression changes with p-values and log fold changes. Image F contains Venn diagrams comparing differentially expressed genes. Image G includes UMAP plots indicating overlap scores for control, lesional, and non-lesional samples, along with a bubble chart of gene ratios. Image H is a dot plot displaying percent expression and average expression levels across conditions. — Comparative scRNA-seq analysis of Eppk1^−/− and psoriatic keratinocytes (A) UMAP visualization of single-cell RNA-seq data from murine wildtype and Eppk1 ^−/− skin, revealing 8 distinct clusters. (B) UMAP of the keratinocyte subset showing seven subclusters corresponding to stages of keratinocyte differentiation. (C) Dot plot displaying expression of plakin family genes, split by condition into wildtype and Eppk1 ^−/−. Dot size reflects the percentage of expressing cells, and color intensity indicates average expression levels. (D) Volcano plot showing differentially expressed genes (DEGs) between wildtype and Eppk1 ^−/− keratinocytes. Genes significantly upregulated in Eppk1 ^−/− (log₂FC > 0.5, adjusted p < 0.05) are shown with positive fold changes, while downregulated genes exhibit negative fold changes. (E) Volcano plot of DEGs between healthy skin and lesional psoriasis within the keratinocyte subset (based on data shown in Figure 1). Significantly upregulated genes in psoriasis compared to healthy skin have positive log₂FC values, and significantly downregulated genes have negative log₂FC values. (F) Venn diagrams illustrating the overlap of significantly regulated genes in keratinocytes between Eppk1 ^−/−, lesional psoriasis, and non-lesional psoriasis, compared to healthy controls/wildtype. (G) Feature plot showing a module score for genes that are consistently downregulated across Eppk1 ^−/− and human psoriasis datasets (as identified in F), split by condition. (H) Dot plot of individual overlapping downregulated genes (from F) across conditions in the human psoriasis dataset. Dot size reflects the percentage of expressing cells; color intensity reflects average expression. (I) Enrichment analysis of overlapping downregulated genes in Eppk1 ^−/− and psoriatic keratinocytes. Top 13 enriched pathways are shown with corresponding combined scores and gene ratios.