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. 2025 Jul 17;13:1617737. doi: 10.3389/fcell.2025.1617737

FIGURE 4.

Grouped UMAP plots and bubble plots analyzing immune and keratinocyte subsets in control versus atopic dermatitis. Panels A-C show cell type distributions and UMAP scores. Panels D and H depict expression levels of various genes, with highlighted genes IFNG and EPPK1. Panels E-G illustrate detailed keratinocyte subsets. Color scales indicate expression and score intensities.

Comprehensive scRNAseq analysis of plakin regulation in atopic dermatitis (A) UMAP visualization from a scRNAseq analysis, revealing 5 distinct cell clusters. (B,C) Feature plots illustrate an atopic dermatitis module score, including the expression levels of CCL27, CD74, IFITM3, IFNG, KRT6A, S100A7, S100A8, S100A9 and SERPINB4 genes. Feature plots are split by condition into (B) healthy skin and (C) atopic dermatitis (AD). (D) Dot plot of AD-related genes split by condition. Dot size represents the expression percentage for each gene with color intensity reflecting average gene expression levels. (E) UMAP of a subset from the keratinocyte cluster, showing 4 clusters representing stages of keratinocyte differentiation. (F,G) Feature plots showing a plakin module score, including expression levels of DSP, DST, EPPK1, EVPL, MACF1, PLEC, and PPL genes, split by condition into (F) healthy skin and (G) atopic dermatitis (AD). (H) Dot plot of epidermal plakins in the keratinocyte subset split by condition. Dot size represents the expression percentage for each gene with color intensity reflecting average gene expression levels.