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. 2005 Sep;73(9):5961–5977. doi: 10.1128/IAI.73.9.5961-5977.2005

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Copyright © 2005, American Society for Microbiology

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FIG. 9. — Effects of rcsB on the expression of selected genes involved in Ysa type III protein secretion. RcsB affects the expression of the genes involved in Ysa TTS. (A) ysaE-lacZYA. (B) orf6 orf7-lacZYA. (C) ysaR-lacZYA. (D) ysaU-lacZYA. (E) ysrRS-lacZYA. (F) sycB-lacZYA. For each panel, levels of expression are displayed for the following different genetic backgrounds: wild type (WT), rcsB (rcsB), plasmid control (rcsB, pTM100), and in trans complementation (rcsB prcsB⁺). β-Galactosidase activity was determined for each strain cultivated in L medium plus 290 mM NaCl at 26°C. The results are averages ± standard deviations from at least two independent experiments performed in duplicate.