TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant feature(s) | Source or reference |
|---|---|---|
| Strains | ||
| Y. enterocolitica | ||
| JB580v | Serogroup O:8, Nalr ΔyenR (R− M+) | 21 |
| GY1087 | yscR::mTn5-Km2 ysaV::pEP185.2 | 39 |
| GY4428 | ysaV::pEP185.2 | This study |
| GY4478 | pYV8081− | 39 |
| GY4531 | crp::str | 32 |
| GY4572 | ysaU::TnMod-lacZYA-RKm′ | This study |
| GY4573 | orf6 orf7::TnMod-lacZYA-RKm′ | This study |
| GY4574 | ddhB::TnMod-lacZYA-RKm′ | This study |
| GY4613 | rcsB::TnMod-lacZYA-RKm′ | This study |
| GY4639 | ysaR::TnMod-lacZYA-RKm′ | This study |
| GY4641 | ysaE::TnMod-lacZYA-RKm′ | This study |
| GY4664 | ysaU::TnMod-lacZYA-RKm′ | This study |
| GY4665 | ysaC::TnMod-lacZYA-RKm′ | This study |
| GY4668 | orf6 orf7::TnMod-lacZYA-RKm′ | This study |
| GY4675 | ysaU::TnMod-lacZYA-RKm′ ysrS::pEP185.2 | This study |
| GY4676 | ysaU::TnMod-lacZYA-RKm′ rcsB::pEP185.2 | This study |
| GY4677 | ysaR::TnMod-lacZYA-RKm′ ysrS::pEP185.2 | This study |
| GY4678 | ysaR::TnMod-lacZYA-RKm′ rcsB::pEP185.2 | This study |
| GY4684 | pYV8081−ysaV::pEP185.2 | This study |
| GY4737 | rcsB::pEP185.2 | This study |
| GY4742 | ysrS::mTn5-Km2-lacZYA | This study |
| GY4887 | ysrS::pEP185.2 | This study |
| GY4888 | ysaE::TnMod-lacZYA-RKm′ rcsB::pEP185.2 | This study |
| GY4891 | orf6 orf7::TnMod-lacZYA-RKm′ rcsB::pEP185.2 | This study |
| GY4892 | ysaE::TnMod-lacZYA-RKm′ ysrS::pEP185.2 | This study |
| GY4893 | rcsB::TnMod-lacZYA-RKm′ ysrS::pEP185.2 | This study |
| GY4894 | ysrS::pFUSE (ysrS-lacZYA) | This study |
| GY4899 | orf6 orf7::TnMod-lacZYA-RKm′ ysrS::pEP185.2 | This study |
| GY5019 | sycB::mTn5-Km2-lacZYA | This study |
| GY5022 | sycB::mTn5-Km2-lacZYA ysrS::pEP185.2 | This study |
| GY5021 | sycB::mTn5-Km2-lacZYA rcsB::pEP185.2 | This study |
| GY5124 | rcsB::aacC1 Gmr | This study |
| GY5196 | rcsB::aacC1 ysrS::pFUSE (ysrS-lacZYA) | This study |
| YVM356 | yscR::mTn5-Km2 | 9 |
| E. coli S17-1 λpir | recA thi pro hsdR−hsdM+ RP4::2-Tc::Mu::Km Tn7 λpir | 27 |
| Plasmids | ||
| p34s-Gm | Gmr plasmid with antibiotic resistance cassette, accC1 | 11 |
| pCR-Blunt II-TOPO | Kmr | Invitrogen |
| pEP185.2 | Cmr, suicide vector | 21 |
| pFUSE | Cmr, suicide vector for transcriptional fusions to lacZYA | 1 |
| pTM100 | mob+oriR6K Tetr Cmr | 24 |
| pTnMod-lacZYA-RKm′ | TnMod-lacZYA-RKm′ in a delivery vector, Kmr | Andrew Darwin, NYU |
| pREV10 | mTn5-Km2-lacZYA′ in a delivery vector, Kmr | Virginia Miller, Washington University |
| pGY100 | Pcat-yplAB derivative of pTM100 | 39 |
| pGY348 | Ca. 0.9-kb fragment of ysaV in pCR-Blunt II-TOPO | This study |
| pGY352 | Ca. 0.9-kb fragment of ysaV in pEP185.2 | This study |
| pGY431 | Ca. 0.5-kb fragment of rcsB in pCR-Blunt II-TOPO | This study |
| pGY435 | Ca. 0.5-kb fragment of rcsB in pEP185.2 | This study |
| pGY454 | ysrRS in pCR-Blunt II-TOPO | This study |
| pGY482 | ysrRS in pTM100 | This study |
| pGY512 | Ca. 0.5-kb fragment of ysrS in pCR-Blunt II-TOPO | This study |
| pGY526 | Ca. 0.5-kb fragment of ysrS in pEP185.2 | This study |
| pGY529 | Ca. 1-kb fragment of ysrS in pCR-Blunt II-TOPO | This study |
| pGY530 | Ca. 1-kb fragment of ysrS in pFUSE | This study |
| pGY551 | rcsB in pCR-Blunt II-TOPO | This study |
| pGY552 | ysrRS in pTM100 with cat removed | This study |
| pGY553 | rcsB in pTM100 | This study |
| pGY556 | pTM100 with cat removed | This study |
| pGY557 | rcsB::accC1 in pCR-Blunt II-TOPO | This study |
| pGY561 | rcsB::accC1 in pEP185.2 | This study |