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. 2005 Sep;73(9):5767–5774. doi: 10.1128/IAI.73.9.5767-5774.2005

FIG. 1.

FIG. 1.

Serum is lethal to wild-type cells in the presence of calcineurin inhibitors, and toxicity is not unique to fetal bovine serum. (A) The reference strain (SC5314) (⧫), a marker-matched strain (DAY185) (▪), a cnb1/cnb1 calcineurin B mutant strain (JRB64) (▴), and a calcineurin B-complemented strain (MCC85) (×) were grown overnight in fetal bovine serum supplemented with FK506 at the indicated concentrations. (B and C) The reference strain (SC5314) (WT), the cnb1/cnb1 calcineurin B mutant strain (JRB64), and the cnb1/cnb1+CNB1 calcineurin B-complemented strain (MCC85) were tested for survival in 100% fetal bovine, porcine, ovine (B), or murine (C) sera. Approximately 5,000 cells/ml of each strain were incubated in the indicated media for 9 h (B) or 48 h (C) at 30°C.