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. 2005 Sep;73(9):5767–5774. doi: 10.1128/IAI.73.9.5767-5774.2005

FIG. 2.

FIG. 2.

The lethal factor of serum is not a protein or a lipid. (A) The wild-type strain (SC5314) (solid bar), a cnb1/cnb1 calcineurin B mutant (JRB64) (shaded bar), and a cnb1/cnb1+CNB1 calcineurin B-complemented strain (MCC85) (open bar) were tested for survival at 9 hours in fetal bovine serum that had been filtered through a 3-kDa Centricon filter. (B) The survival of wild-type (▪), cnb1/cnb1 (▴), and cnb1/cnb1+CNB1 (×) strains was measured at the indicated times in the soluble fraction of fetal bovine serum that had been boiled and centrifuged to denature and remove proteins. (C) The survival of wild-type (solid bars), cnb1/cnb1 (shaded bars), and cnb1/cnb1+CNB1 (open bars) strains in YPD, proteinase K-pretreated YPD (YPD-PK), serum, or proteinase K-pretreated serum (serum-PK) was measured at 24 h. (D) Survival of wild-type (solid bars), cnb1/cnb1 (shaded bars), and cnb1/cnb1+CNB1 (open bars) strains was measured at 24 h in chloroform-extracted serum, serum, chloroform-extracted YPD, or YPD that had been lyophilized and reconstituted.