FIG. 4.

Calcineurin is important for the response to stress induced by Ca²⁺ in serum. (A) The abilities of wild-type (SC5314) (solid bars), cnb1/cnb1 (JRB64) (shaded bars), and cnb1/cnb1+CNB1 (MCC85) (open bars) strains to persist in PBS were measured with or without 5 mM Ca²⁺. (B) Wild-type (▪), cnb1/cnb1 (▴), and cnb1/cnb1+CNB1 (×) strains were tested for their abilities to survive and proliferate in serum alone or in serum supplemented with the indicated concentrations of BAPTA. (C) The survival of the cnb1/cnb1 calcineurin mutant strain was measured in serum with 0.8 mM BAPTA and supplemented with the indicated concentrations of Ca²⁺. Cells were incubated at 30°C with (A) or without (B and C) agitation. The experiments for which results are shown in panels B and C were performed in 96-well plates.