FIG. 2.
Construction of B. cenocepacia BC7 mutants in the 22-kDa adhesin and cbl pilus operon. (A) Physical map of the B. cenocepacia adhA gene with insertion of the trimethoprim resistance cassette. The adhA gene in BC7 corresponds to nucleotide numbers 2383022 through 2391644 on chromosome 2 in J2315. The adhA gene was interrupted at the AgeI site (344 bp from the start of translation) with a trimethoprim resistance (Tpr) cassette in the B. cenocepacia BC7 chromosome. The large arrow indicates the direction of adhA transcription. Primers generated for gene replacement strategy (adhA-1 and adhA-2) are represented by directional arrows. Asterisks indicate repeats with deletions or truncations. The scale legend corresponds to the size of an intact repeat unit. (B) Physical map of the cbl operon with insertion of trimethoprim resistance cassettes. Arrows indicate the direction of transcription. cblA and cblS were interrupted individually in the B. cenocepacia BC7 chromosome with a trimethoprim resistance cassette.