FIG. 3.

Numbers of M. gallisepticum-specific IgG (A)- and IgA (B)-secreting cells per million leukocytes isolated from the tracheas of GT5- or PBS-vaccinated chickens postchallenge as measured by ELISPOT assay. The cells isolated from tracheas of two birds within the same group were pooled on day 1 postchallenge in order to acquire sufficient cell numbers. GT5-vaccinated chickens had significantly higher numbers of specific IgG- and IgA-secreting B/plasma cells. Bars represent the means ± standard errors of the means for four birds at each time point. *, statistical significance at P < 0.05.