FIG. 4.

M. haemolytica LKT downregulates the antiapoptotic proteins Bcl-2 and Bcl_XL. BL-3 cells were incubated with LKT (0.2 U) for the designated time periods at 37°C. Cells were washed three times with RPMI, lysed with Triton-X lysis buffer at 4°C for 20 min, and Western immunoblotting was performed for Bcl-2, Bcl_XL, and β-actin. (B) Images shown were quantified with a densitometer; the data are illustrated as means ± SEM of three separate experiments (*, P < 0.05).