TABLE 1.
Bacterial strains, plasmids, and oligonucleotide primers
| Strain, plasmid, or primer | Characteristic(s) or relevant feature(s) | Source or reference |
|---|---|---|
| Brucella strains | ||
| 1330 | B. suis biotype 1, wild type, ATCC 23444T | American Type Culture Collection |
| 1330-GFP | gfp insertion into chromosomal DNA of B. suis 1330, Cmr Nalr | 19 |
| 3G1 | 1330-GFP with pBBR1-3G1 plasmid | This work |
| 1330bvfA::kan | 1330 Kanr; mutant of bvfA gene | This work |
| 1330bvfA::kan(pBBR1-BvfA) | 1330bvfA::kan with pBBR1-BvfA plasmid | This work |
| Plasmids | ||
| pBBR1-YopP | pBBR1MCS4 (22) containing a promoterless yopP | This work |
| pBBR1-3G1 | pBBR1-YopP with BvfA-YopP fusion | This work |
| pBBR1-BvfA | pBBR1MCS containing bvfA gene | This work |
| pBBR1-KGFP | pBBR1MCS containing a promoterless gfpmut3 | 24 |
| pBBR1-KGFPvirB | pBBR1-KGFP with gfpmut3 controlled by pvirB | 5 |
| pBBR1-KGFPcons | pBBR1-KGFP with gfpmut3 controlled by a constitutive promoter | 24 |
| pBBR1-KGFPbvfA | pBBR1-KGFP with gfpmut3 controlled by pbvfA | This work |
| PCR primers (5′-3′)a | ||
| YopEcoU | CGGAATTCGGTGGCTTATCAGAAAAA | This work |
| YopXbaL | GCTCTAGACCATGAGCCAGAAAGAACT | This work |
| BvfA-F | ACCCTTCGTCGATGTGCTGA | This work |
| BvfA-L | CCGCGCTGATTTCATCGCTG | This work |
| PSKU | GCGGTACCCTTCGTCGATGTGCTGA | This work |
| PSBL | GCGGATCCAGAAAAGCCAGGGAATTGA | This work |
a
The restriction sites are underlined.