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IFN-γ production in PBMC and PBMC depleted of NK cells stimulated with mycobacterial antigens. The samples were stimulated with MPP14 (2 μg/ml), ESAT-6 (2 μg/ml), or PPD (10 μg/ml) for 24 h and the supernatant was assayed for IFN-γ production. The results are the means of cells stimulated in triplicates ± standard error of the mean. The experiment was repeated three times using PBMC from six different animals.
