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Iron depletion inhibits biofilm formation in N16961. Planktonic growth and biofilm formation were measured as described in Materials and Methods and are expressed as percentages of the growth or biofilm produced in EZ RDM without added iron or chelator. Means and standard deviations of three independent experiments are shown. (A) Planktonic growth of N16961 in the biofilm assay with or without added iron or the iron chelator EDDA. (B) Biofilm formation by the cultures shown in panel A.
