TABLE 2.
Oligonucleotide primers used in this study
| Name | Primer sequence (5′-3′)a |
|---|---|
| bfd1 | CGTTTGTTTGTGTCATGGTGTCTC |
| bfd2 | TGAGCAATCAGCGATTCTTCG |
| fur1 | TGGGCGTCGACCAGCCAAGAGCC (SalI) |
| fur2 | GTGAGCGTTAACTTTAAGACCAGC (HpaI) |
| fur3 | GGTCTTAAAGTTAACGCTCACAAGCC (HpaI) |
| fur4 | GGAGGCGCTGATATCCATTTGGC (EcoRI) |
| mshA1 | GGGAGATCTTTCAGCGAAAGCGAATAGTGG (BglII) |
| mshA2 | ATAGTCGACCCATTGCACCAGCAACTGCACC (SalI) |
| mshA3 | AAAGTCGACCCTGCAACGGTTGCTATGC (SalI) |
| mshA4 | CCCTCTAGAGTGGTTACCACCGCAAAGG (XbaI) |
| polyT-ClaI | CCATCGATTTTTTTTTTTTTTTTTT (ClaI) |
| sodB1 | ATTTCCACCACGGTAAGCACC |
| sodB2 | CCGAAGTTGTTGATGGCTGAATC |
| vryhB1 | TGTGTCGACTGATGAAACAACGG (SalI) |
| vryhB2 | ATGTCGGTTAACCTTCACTTGTTCCC (HpaI) |
| vryhB3 | AAGTGAAGGTTAACCGACATCTTCGG (HpaI) |
| vryhB4 | TACCGTAGGTCGACATCAACGC (SalI) |
| vryhB5 | GGAATCCATGGTCGAGAGCC (NcoI) |
| vryhB6 | TTCACTGGATCCCTAAGACG (BamHI) |
| vryhB8 | CACGTCTTAGGGAACAAGTG |
| vryhB10 | GCTAAAAATTACACTGGAAGC |
| vryhB12 | AGAATTCACGTCTTAGGGAACAAGTG (EcoRI) |
| vryhB13 | GCCAAGCTTACGAGGTCAAAGCC |
| vryhB14 | TTCAAGCTTGTCCACTCATTGC |
| vryhB19 | ATCGAATTCCTCGATATGCGGTAGCG (EcoRI) |
| vryhB20 | GCTCGATATCAAAACGTTCTACAGC (EcoRV) |
| vryhB21 | CGCATATCGGTTAATAATAATAGTTCTC |
| vryhB22 | TATTATTAACCGATATGCGGTAGCG |
| vryhB23 | TGATGGATCCGCCAGTGGATGAG (BamHI) |
a
Any relevant recognition sites for restriction enzymes (listed in parentheses) are underlined.