TABLE 3.
Genes whose expression is decreased by RyhBa
| Encoded protein | Gene designation(s) (decrease in expression [n-fold]) |
|---|---|
| Products of known RyhB-regulated genes | |
| Superoxide dismutase (SodB)b,c,d | VC2045 (15.6) |
| Succinate dehydrogenaseb,c,d | VC2089 (2.2), VC2090 (2.2), VC2091 (2.0) |
| Fumarasec | VC1304 (2.8) |
| Metabolism, respiration, and other functions | |
| Fumarate reductasec,d | VC2656 (2.5), VC2658 (2.8), VC2659 (6.8) |
| Citrate synthasec | VC2092 (3.3) |
| Ubiquinol-cytochrome c reductaseb,c | VC0573 (2.2), VC0574 (3.6), VC0575 (3.0) |
| RnfEGDB-related proteinsb,d | VC1012 (2.2), VC1013 (2.0), VC1014 (2.0), VC1016 (2.0) |
| Cytochrome c oxidase (CcoPQRN)b,c | VC1439 (2.7), VC1440 (2.5), VC1441 (2.0), VC1442 (2.0) |
| Cytochrome d oxidasec | VC1843 (9.7), VC1844 (2.4) |
| Cytochrome c-type biogenesis proteinb | VC2052 (2.7) |
| Cytochrome b561b | VCA0249 (2.6) |
| 2-Oxoglutarate dehydrogenase E2 E1b | VC2086 (2.0), VC2087 (2.0) |
| NADH-ubiquinone oxidoreductaseb,c,d | VC2205 (2.1), VC2290 (2.0), VC2291 (2.0), VC2292 (2.0) |
| Oxaloacetate decarboxylaseb | VC0550 (2.3) |
| NADP transhydrogenasec,d | VCA0564 (2.0) |
| OmpUb | VC0633 (3.3) |
| OmpWc | VCA0867 (2.8) |
| Maltose ABC transporterc,d | VCA0944 (4.4), VCA0945 (2.5) |
| Maltose operon periplasmic proteinc,d | VCA1027 (3.4), VCA1028 (4.1) |
| Formate transporterb | VCA0540 (2.0) |
| A/G-specific adenine glycosylaseb | VC0452 (4.0) |
| Iron-regulated protein | |
| Hemolysinb | VCA0219 (4.1) |
| Hypothetical proteins | |
| VC1559b (3.9) | |
| VCA0377c (3.8) | |
| VCA0502c (2.1) | |
| VCA0505c (2.5) | |
| VCA0651b (2.3) |
a
RNAs were isolated from the parental strain and the ryhB mutant or from the ryhB mutant and the ryhB overexpression strain grown to late log phase and analyzed using microarrays.
b
Mean expression of gene was at least twofold higher in the ryhB mutant of N16961 than in the wild-type parental strain in three arrays.
c
Mean expression of gene was at least twofold lower in the ryhB mutant of O395 overexpressing ryhB than in the ryhB mutant carrying the vector in three arrays.
d
Mean expression of gene was at least twofold higher in the ryhB mutant of O395 than in the wild-type parental strain in three arrays.