TABLE 2.
Respiratory burst of PMNs exposed to UPEC
| Strain | Nitroblue tetrazolium reductiona (% positive)
|
Luminol enhanced chemiluminescenceb
|
||
|---|---|---|---|---|
| 15 min | 30 min | Peak relative light units | Time to peak (min) | |
| CP9(pSX34LacZα) | 80 (79-83) | 90 (87-97) | 970 (654-1287) | 47.5 (37.1-57.9) |
| CP9cnf1(pSX34LacZα) | 62 (60-63)c | 77 (67-77)d | 452 (389-514)e | 57.5 (33.5-81.5) |
| CP9cnf1(pHLK140) | 87 (81-89) | 94 (85-96) | 920 (672-1168) | 41.2 (36.7-45.6) |
a
Data are the median and range of three independent experiments, and strains are designated as follows: wild-type [CP9(pSX34LacZα)], mutant [CP9cnf1(pSX34LacZα)], and complemented mutant [CP9cnf1(pHLK140)].
b
Values are shown as the mean and 95% confidence interval of three experiments in duplicate.
c,d
c,d Significantly less than wild-type or complemented mutant (P = 0.05), Mann-Whitney test.
e
Significantly less than wild-type and complemented mutant (P < 0.01) as determined by analysis of variance.